Estragole DNA adduct accumulation in human liver HepaRG cells upon repeated in vitro exposure
نویسندگان
چکیده
منابع مشابه
From in vitro to in vivo: Integration of the virtual cell based assay with physiologically based kinetic modelling
Physiologically based kinetic (PBK) models and the virtual cell based assay can be linked to form so called physiologically based dynamic (PBD) models. This study illustrates the development and application of a PBK model for prediction of estragole-induced DNA adduct formation and hepatotoxicity in humans. To address the hepatotoxicity, HepaRG cells were used as a surrogate for liver cells, wi...
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Drug-induced human hepatotoxicity is difficult to predict using the current in vitro systems. In this study, long-term 3D organotypic cultures of the human hepatoma HepaRG cell line were prepared using a high-throughput hanging drop method. The organotypic cultures were maintained for 3 weeks and assessed for (1) liver specific functions, including phase I enzyme and transporter activities, (2)...
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ژورنال
عنوان ژورنال: Toxicology Letters
سال: 2021
ISSN: 0378-4274
DOI: 10.1016/j.toxlet.2020.11.009